Journal: Insect Science

Article Title: lncR26319/miR‐2834/ EndophilinA axis regulates oogenesis of the silkworm, Bombyx mori

doi: 10.1111/1744-7917.13082

Figure Lengend Snippet: List of primers

Article Snippet: The lncR26319 ASO was transfected into the BmN4 cells with liposome (FuGENE ® HD Transfection Reagent, Promega, Madison, WI, USA).

Techniques: Negative Control, Sequencing, Control, Mutagenesis, In Situ Hybridization

Journal: Insect Science

Article Title: lncR26319/miR‐2834/ EndophilinA axis regulates oogenesis of the silkworm, Bombyx mori

doi: 10.1111/1744-7917.13082

Figure Lengend Snippet: lncR26319 regulates Endophilin A ( EndoA ) by binding to miR‐2834. The expression level of lncR26319 (A) and miR‐2834 (B) after knockdown of lncR26319 with antisense oligonucleotide (ASO) (lncR26319i). (C) Expression change of EndoA after lncR26319 knockdown (lncR26319i). (D) Messenger RNA (mRNA) level of lncR26319 after overexpression of lncR26319 (pIE‐EGFP‐lncR26319). (E) Expression change of EndoA after lncR26319 and miR‐2834 overexpress in BmN4 cells. Three independent experiments were performed, and each sample was repeated 3 times (values are means ± SEM of 3 experiments. * P < 0.05, *** P < 0.001). Significance analysis was conducted with ANOVA and Student's t ‐test. ANOVA, analysis of variance; SEM, standard error of the mean.

Article Snippet: The lncR26319 ASO was transfected into the BmN4 cells with liposome (FuGENE ® HD Transfection Reagent, Promega, Madison, WI, USA).

Techniques: Binding Assay, Expressing, Knockdown, Over Expression

Journal: Insect Science

Article Title: lncR26319/miR‐2834/ EndophilinA axis regulates oogenesis of the silkworm, Bombyx mori

doi: 10.1111/1744-7917.13082

Figure Lengend Snippet: Expression changes of yolk family genes after knockdown of miR‐2834 in BmN4 cells. Expression changes of miR‐2834 (A), lncR26319 (B), and Endophilin A ( EndoA ) (C) after knockdown of miR‐2834. Expression level of yolk protein (Yp) genes were normalized to actinA3 . (D) Expression changes of yolk family genes after repression of miR‐2834. The expression level of yolk family genes was normalized to RP49 . Three independent experiments were performed, and each sample was repeated 3 times (values are means ± SEM of 3 experiments. n.s. P > 0.05, * P < 0.05, *** P < 0.001). Significance analysis was conducted with ANOVA and Student's t ‐test. ANOVA, analysis of variance; SEM, standard error of the mean.

Article Snippet: The lncR26319 ASO was transfected into the BmN4 cells with liposome (FuGENE ® HD Transfection Reagent, Promega, Madison, WI, USA).

Techniques: Expressing, Knockdown